Sub-chronic Toxicity Evaluation of Ethyl Acetate Fraction of Fruit Rind of “Asam Kandis” (Garcinia Cowa Roxb.) Against Liver and Kidney Function of Female White Mice

The sub-chronic toxicity testing of ethyl acetate fraction of fruit rind of “asam kandis” (Garcinia cowa Roxb.) to the liver and kidney function has been carried out to female white mice. A total of 18 female white mice aged 2-3 months weighing 20-30 grams are used as test animals. Animals were divided into three groups: one control group and two treatment groups. Groups were given daily ethyl acetate fraction at the doses of 500 and 1000 mg/kg orally for 60 days. Parameters observed were the activity of SGPT and liver weight ratio to observe the liver function; serum creatinine level and kidney weight ratio to determine kidney function. Data of SGPT, serum creatinine and the weight ratio of liver and kidney were analyzed by two-way ANOVA. Results show that the activity of SGPT and serum creatinine level were directly affected by the dose (p0.05). The study concluded that the dosage of ethyl acetate fraction of the fruit rind of G. cowa had a significant effect on the activity of SGPT and serum creatinine level of female white mice. The duration of administration did not give a significant effect on the increase of serum creatine level as well as the organ weight ratio of liver and kidney of mice